Journal: Cancer Research

Article Title: Repurposing Ceritinib Induces DNA Damage and Enhances PARP Inhibitor Responses in High-Grade Serous Ovarian Carcinoma

doi: 10.1158/0008-5472.can-21-0732

Figure Lengend Snippet: Figure 5. Combination effects of ceritinib and olaparib in HGSOC PDX models. A–E, SCID beige mice were inoculated by intraperitoneal injection of disaggregated PDX tumors, with indicated gene alterations and HRD scores. When tumors reached 0.3–0.5 cm2 in cross-sectional area by transabdominal ultrasound, mice were treated daily by oral gavagewith 100 mg/kg ceritinib,50 mg/kg olaparib,or100 mg/kg ceritinib þ50 mg/kg olaparib for 9weeks or until tumor dimensions determined byultrasound indicated that tumors were greater than or equal to 10% of body weight or if humane endpoints were met. Tumor areas were monitored weekly by transabdominal ultrasound. Coloring in the plot indicates drug arm. Predicted lines are the average estimates computed from the statistical model fixed effects, relative to the arm- specific baseline estimate. Shading indicates 95% confidence intervals. The P values are provided in Supplementary Table S3. The number of mice under observation at each time point for each arm is indicated below the x-axis as a function of time, where text color indicates drug arm.

Article Snippet: Tumors were established by intraperitoneal injection into female SCID beige mice (C.B.-17/IcrHsd-Prkdcscid Lystbg; ENVIGO) as previously described (18).

Techniques: Injection

Journal: Cancer Science

Article Title: CCL2 induces resistance to the antiproliferative effect of cabazitaxel in prostate cancer cells

doi: 10.1111/cas.13876

Figure Lengend Snippet: The CCR2 antagonist restored the sensitivity to cabazitaxel in vivo. A, After 2 wk of acclimatization, 2 × 10 6 DU145 cells were implanted subcutaneously in SCID mice. The control group was intraperitoneally injected with 20 μ L of DMSO, and the cabazitaxel group was intraperitoneally injected with cabazitaxel weekly (days 0, 7 and 14) at a dose of 7 mg/kg diluted with 20 μ L of DMSO (n = 5). The tumor size was measured every other day using a caliper. B, Body weight was measured every other day using a scale. C, After 2 wk of acclimatization, 2 × 10 6 DU145‐TxR/CxR cells were implanted subcutaneously in SCID mice. The following groups were compared: control, cabazitaxel alone, CCR2 antagonist alone, and a combination of cabazitaxel and CCR2 antagonist. The control group was injected with 20 μ L of DMSO. Cabazitaxel was injected weekly (days 0, 7 and 14) at a dose of 7 mg/kg, and the CCR2 antagonist was injected every other day at a dose of 50 μ g/kg (n = 6). The left, middle and right bars on the right side of the graph illustrate the comparison between the combination group and the CCR2 antagonist group, the cabazitaxel group and the control group, respectively. The tumor size was measured every other day using a caliper. D, On day 21, the mice were killed and the tumors extracted. E, Body weight was measured every other day using a scale. Data are shown as means ± SEM

Article Snippet: Intact male SCID mice (aged 6‐7 weeks) were obtained from CLEA Japan (Tokyo, Japan).

Techniques: In Vivo, Control, Injection, Comparison